The thrombin time (TT), also known as the thrombin clotting time (TCT) is the time taken for a clot to form when thrombin is added to platelet-poor plasma.
No phospholipid or calcium is required for the test. As the reagent is thrombin, the test is not sensitive to deficiencies in thrombin.
The TT is sensitive to thrombin inhibitors, hypofibrinogenaemia, dysfibrinogenaemia, presence of fibrin degradation products, hypoalbuminaemia and other substances which impair fibrin formation, such as the presence of paraproteins.
TT Method
The TT assay requires patient platelet poor plasma (PPP) and a thrombin reagent. Timing starts when thrombin is added to PPP and stops when a clot forms, preventing light transmittance to the optical detector.
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